Anti-HLA Class I Antibody [MEM-147] (A85472)

Anti-HLA Class I Antibody [MEM-147]

Mouse monoclonal [MEM-147] antibody to HLA Class I.

The antibody recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Flow Cytometry, IP, WB

Flow Cytometry: 1-5 µg/ml, WB: Non-reducing conditions.

Human

PHA-activated peripheral blood lymphocytes.

Mouse

Monoclonal

MEM-147

Protein A chromatography.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.