The antibody recognises MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.
Applications
Flow Cytometry, IP, IHC-Fr, ICC, ELISA, CyTOF
Dilutions
Flow Cytometry: 1-12 µg/ml
Reactivity
Bovine, Human, Non-Human Primates, Feline
Cross Reactivity
This antibody does not cross react with Rabbit.
Immunogen
Membrane of human tonsil cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
W6/32
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)
Flow cytometry surface staining pattern of human peripheral whole blood labeling HLA Class I with Anti-HLA Class I Antibody [W6/32] (A85488), (concentration in sample 4 µg/ml, GAM APC).
Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)
Separation of leukocytes stained with Anti-HLA Class I Antibody [W6/32] (A85488), (concentration in sample 4 µg/ml, GAM APC, red-filled) from leukocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
Publishing research using Anti-HLA Class I Antibody [W6/32] (A85488)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-HLA Class I Antibody [W6/32] (A85488)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)](https://cdn.antibodies.com/image/catalog/85/A85488_1.jpg?profile=product_top)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)](https://cdn.antibodies.com/image/catalog/85/A85488_2.jpg?profile=product_top)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)](https://cdn.antibodies.com/image/catalog/85/A85488_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)](https://cdn.antibodies.com/image/catalog/85/A85488_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)](https://cdn.antibodies.com/image/catalog/85/A85488_1.jpg?profile=product_image)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] (A85488)](https://cdn.antibodies.com/image/catalog/85/A85488_2.jpg?profile=product_image)
![Flow Cytometry - Anti-HLA Class I Antibody [W6/32] - Low endotoxin, Azide free (A85491)](https://cdn.antibodies.com/image/catalog/85/A85491_1.jpg?profile=product_alternative)
![WB - Anti-HLA Class I Antibody [MEM-147] (A85472)](https://cdn.antibodies.com/image/catalog/85/A85472_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-HLA Class I Antibody [MEM-123] (A85507)](https://cdn.antibodies.com/image/catalog/85/A85507_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-HLA Class I Antibody [MEM-81] (A86249)](https://cdn.antibodies.com/image/catalog/86/A86249_1.jpg?profile=product_alternative)
