Human Angiotensinogen ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Angiotensinogen in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Angiotensinogen ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human AGT in the samples. A monoclonal antibody specific for human AGT has been pre-coated onto a microplate. Standard, samples and HRP-linked detect antibody specific for AGT are pipetted into the wells and AGT present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution is added to the wells and color develops in proportion to the amount of AGT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
19.93 pg/ml
Product Range
312.50 - 20000 pg/ml
Specificity
This kit can assay both native and recombinant human AGT.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Storage
Store at +4°C. Do not use past expiration date!
Synonyms
AGT, Serpin A8, SERPINA8
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
