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Human Anti-FSH Antibody ELISA Kit (A302999)

$815

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-11 business days.
Name
Human Anti-FSH Antibody ELISA Kit
Description
Human Anti-FSH Antibody ELISA Kit is an indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Anti-FSH Antibody in serum, plasma, tissue homogenates, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Anti-FSH Antibody ELISA Kit (A302999) employs HRP antibody sandwich ELISA for the quantitative measurement of human Anti-FSH Antibody in serum, plasma, tissue homogenates, and other biological fluids. Capture antibody specific for Anti-FSH Antibody is pre-coated on a 96-well microtiter plate. Samples and standards are added, and Anti-FSH Antibody binds to the immobilized antibody. Detection is performed using HRP-conjugated detection antibody, which binds to a different epitope on the captured target. Following another wash, TMB substrate solution produces a blue-coloured product that changes to yellow after addition of acidic stop solution. The intensity of yellow is proportional to the amount of Anti-FSH Antibody captured, and the concentration is determined by reading O.D. at 450nm.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Sensitivity
0.938 ng/ml
Product Range
1.563-100 ng/ml
Assay Time
3 hours
Reactivity
Human
Recovery
Sample Type n Range Average
Serum 5 86% - 103% 92%
EDTA Plasma 5 90% - 99% 93%
Heparin Plasma 5 91% - 96% 93%
Linearity
Sample Type n 1:2 1:4 1:8
Serum 5 86-99% 86-105% 87-99%
EDTA Plasma 5 82-92% 82-100% 83-101%
Heparin Plasma 5 80-87% 82-93% 87-96%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Storage
Store at +4°C. Do not use past expiration date!
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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