Principle of Assay
Human H3cit-NE/Neutrophil Extracellular Trap ELISA Kit (A319528) employs an enzyme immunoassay for the qualitative detection of human H3cit-NE/Neutrophil Extracellular Trap in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate is coated with capture antibodies or antigens specific for H3cit-NE/Neutrophil Extracellular Trap. Samples are added and incubated, followed by washing and addition of enzyme-conjugated detection molecules. TMB substrate is added to produce a blue-coloured product that turns yellow after acidic stop solution. The presence or absence of yellow color indicates the presence or absence of H3cit-NE/Neutrophil Extracellular Trap in the sample.
