Principle of Assay
Human Hepatitis B Virus Pre-S1 Antigen ELISA Kit (A75125) is a 3 hour qualitative Enzyme-Linked Immunosorbent Assay (qELISA) designed for the in vitro qualitative detection of human Hepatitis B Virus Pre-S1 Antigen in serum, plasma, tissue homogenates, and other biological fluids. Samples are added to a 96-well microtiter plate pre-coated with assay-specific binding reagents. Following incubation, unbound components are removed by washing and an enzyme-conjugated detection reagent is added. After further incubation and washing, TMB substrate solution is added to visualize the enzyme-mediated reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The presence or absence of colour indicates the presence or absence of Hepatitis B Virus Pre-S1 Antigen in the sample.