Mouse monoclonal [TB28-2] antibody to Human Kappa Light Chain (PE).
Specificity
This antibody recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It also detects free kappa light chains.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Extracellular and Intracellular staining.
Reactivity
Human
Immunogen
Human IgG-kappa myeloma protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
TB28-2
Isotype
IgG1
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human Ig Lambda Kappa Chain positive B cells (red-filled) from Ig Kappa Light Chain negative B cells (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling Human Kappa Light Chain with Anti-Human Kappa Light Chain Antibody [TB28-2] (PE) (A86591), (10 µl reagent / 100 µl of peripheral whole blood).
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