Human Layilin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Layilin in cell culture supernatant, serum and plasma.
Assay Type
Sandwich (quantitative)
Principle of Assay
Human Layilin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human LAYN in the samples. A monoclonal antibody specific for human LAYN has been pre-coated onto a microplate. Standard, samples and HRP-linked detect antibody specific for LAYN are pipetted into the wells and LAYN present is bound by the immobilized antibody and detect antibody following incubation. After washing, substrate solution is added to the wells and color develops in proportion to the amount of LAYN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Cell culture supernatant, serum and plasma.
Sensitivity
4.25 pg/ml
Product Range
31.25 - 2000 pg/ml
Specificity
This kit can assay both native and recombinant human LAYN.
Reactivity
Human
Cross Reactivity
This ELISA kit has shown no significant cross-reactivity.
Recovery
97% (Average Recovery from Serum Samples).
Storage
Store at +4°C. Do not use past expiration date!
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
