Human Anti-Luteinizing Hormone Antibody ELISA Kit (A302998)

Human Anti-Luteinizing Hormone Antibody ELISA Kit

Human Anti-Luteinizing Hormone Antibody ELISA Kit is a 3 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Anti-Luteinizing Hormone Antibody in serum, plasma, tissue homogenates, and other biological fluids.

Sandwich (quantitative)

Human Anti-Luteinizing Hormone Antibody ELISA Kit (A302998) is a 3 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Anti-Luteinizing Hormone Antibody in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Anti-Luteinizing Hormone Antibody. Standards and samples are added to the wells, where Anti-Luteinizing Hormone Antibody binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody specific for Anti-Luteinizing Hormone Antibody is added to the wells. The detection antibody binds the captured Anti-Luteinizing Hormone Antibody to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-Luteinizing Hormone Antibody captured in each well. The concentration of Anti-Luteinizing Hormone Antibody is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

1.563-100 ng/mL

3 hours

Human

Demonstrates specificity for anti-Luteinizing Hormone antibodies with no reported cross-reactivity to related analogs.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.