Human Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM ELISA Kit (A319451)

Human Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM ELISA Kit

Human Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM ELISA Kit is an indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of human Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM in serum, plasma, tissue homogenates, and other biological fluids.

Indirect (quantitative)

Human Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM ELISA Kit (A319451) employs the indirect sandwich enzyme immunoassay technique for the quantitative measurement of human Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM in serum, plasma, tissue homogenates, and other biological fluids. A capture antibody specific for Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM is pre-coated onto the 96-well microtiter plate. Samples and standards are added, and Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM present in each sample binds to the immobilized antibody. After washing, a Biotinylated detection antibody is added and incubated. Following washing, HRP-Streptavidin is added and incubated. TMB substrate is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Anti-SARS-CoV-2 Spike RBD (omicron B.1.1.529) IgM captured, and the concentration is calculated by reading O.D. at 450nm and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma, tissue homogenates, and other biological fluids.

1.563-100 ng/ml

1h 30m

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at +4°C. Do not use past expiration date!

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.