This Hydroxyl Free Radical Scavenging Capacity Assay Kit is a quantitative assay kit for measuring Hydroxyl Free Radical Scavenging Capacity in animal and plant tissues, serum, plasma, cells, bacteria, cell supernatant, juice, and honey.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit is specially developed for the detection of hydroxyl free radical scavenging capacity in various samples. The operation is simple and convenient, and the detection is sensitive and accurate. In this assay, hydrogen peroxide/ Fe2+ generates hydroxyl free radicals through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radicals and reacts with them to produce 2,3-dihydroxybenzoic acid with a maximum absorption peak at 520 nm. The presence of substances with the capacity to scavenge hydroxyl free radicals, will reduce the absorbance at 520 nm. The absorbance at 520 nm will reflect the free radical scavenging capacity of the test sample.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal and plant tissues, serum, plasma, cells, bacteria, cell supernatant, juice, and honey.
Components
Ferrous Salt, Hydrogen Peroxide, Salicylic acid, Free-HSA Negative Control
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
96-well microplate / microglass cuvette is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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