Rabbit polyclonal antibody to I kappaB- alpha (Phospho-Tyr305)
Specificity
The antibody detects endogenous level of I kappaB- alpha only when phosphorylated at Tyrosine 305.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 305(L-P-Y(p)-D-D) derived from Human I kappaB- alpha.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
39kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from COS-7 cells using I kappaB- alpha (Phospho-Tyr305) Antibody #12050.The lane on the left is treated with the antigen-specific peptide.
