This antibody recognizes Ikaros, a transcription factor expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Mouse, Human
Immunogen
Recombinant fragment corresponding to the C terminus of human Ikaros protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
4E9
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
DNA-binding protein Ikaros, IK1, Ikaros family zinc finger protein 1, IKZF1, LYF1, Lymphoid transcription factor LyF-1, ZNFN1A1
Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained with Anti-Ikaros Antibody [4E9] (PE) (A86433), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with Ikaros
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.