Rabbit polyclonal antibody to IkB-a (Phospho-Ser32 / Ser36)
Specificity
The antibody detects endogenous level of IkB-a only when phosphorylated at serine 32 / 36.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of serine 32 / 36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a (Phospho-Ser32/Ser36) Antibody #11152 (left) or the same antibody preincubated with blocking peptide (right).
