Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody (A94552)

Name: Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody
Brand: Antibodies.com
SKU: A94552
Price: 120.0 GBP
Availability: InStock

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$200

Rabbit polyclonal antibody to IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody

Description

Rabbit polyclonal antibody to IKK alpha (phospho Ser176) + IKK beta (phospho Ser177).

Specificity

This antibody detects endogenous levels of IKK-alpha only when phosphorylated at Ser177.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human IKK-alpha around the phosphorylation site of Ser177 (amino acids 151-200).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

84kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody (A94552)

Western blot analysis of lysates from NIH/3T3 cells treated with TNF 20ng/ml 30' using Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunohistochemistry - Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody (A94552)

Immunohistochemical analysis of paraffin-embedded human heart using Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Western Blot - Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody (A94552)

Western blot analysis of NIH 3T3 cells using Anti-IKK alpha (phospho Ser176) + IKK beta (phospho Ser177) Antibody.

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