Rabbit polyclonal antibody to iNOS (Phospho-Tyr151)
Specificity
The antibody detects endogenous level of iNOS only when phosphorylated at Tyrosine 151.
Applications
ELISA. WB, IHC
Reactivity
Human, Rat
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 151 (Q-Y-Y(p)-G-S) derived from Human iNOS.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
HEP-NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, Nitric oxide synthase, inducible, NOS type II, NOS2, NOS2A, Peptidyl-cysteine S-nitrosylase NOS2
Western blot analysis of extracts from Rat kidney using iNOS (Phospho-Tyr151) Antibody #12067.The lane on the right is treated with the antigen-specific peptide.
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