Rabbit polyclonal antibody to iNOS (Phospho-Tyr151)
Specificity
The antibody detects endogenous levels of Inos only when phosphorylated at tyrosine 151.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 151(Q-Y-Y(p)-G-S) derived from Human iNOS .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
HEP-NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, Nitric oxide synthase, inducible, NOS type II, NOS2, NOS2A, Peptidyl-cysteine S-nitrosylase NOS2
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Inos (Phospho-Tyr151) antibody #11766 (left)or the same antibody preincubated with blocking peptide (right).
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