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Anti-Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) Antibody (A91506)

Western Blot - Anti-Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) Antibody (A91506) - Antibodies.com
$515
Product Datasheet

Rabbit polyclonal antibody to Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) Antibody
Description
Rabbit polyclonal antibody to Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around Y1165 & Y1166 of human IGF1RIGF1R (NP_000866.1).
Sequence
TDYYRK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
125 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) Antibody (A91506) - Antibodies.comEnlarge Image

Western Blot - Anti-Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) Antibody (A91506)

Western blot analysis of extracts of HeLa cells, using Anti-Insulin Receptor (phospho Tyr1165 + Tyr1166) + IGF1 Receptor (phospho Tyr1189 + Tyr1190) Antibody (A91506) at 1:1,000 dilution. HeLa cells were treated by IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
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