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Anti-Keratin 8 (phospho Ser432) Antibody (A94572)

Western Blot - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.com Immunohistochemistry - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.com Immunofluorescence - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.com Western Blot - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.com
This product is discontinued

Anti-Keratin 8 (phospho Ser432) Antibody (A94572) has been discontinued and is no longer available.

View all Cytokeratin 8 Antibodies.

  • Specifications

Name
Anti-Keratin 8 (phospho Ser432) Antibody
Description
Rabbit polyclonal antibody to Keratin 8 (phospho Ser432).
Specificity
This antibody detects endogenous levels of Keratin 8 only when phosphorylated at Ser432.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Keratin 8 around the phosphorylation site of Ser432 (amino acids 401-450).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
53kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CK-8, CYK8, Cytokeratin-8, K8, Keratin, type II cytoskeletal 8, Keratin-8, KRT8, Type-II keratin Kb8
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.comEnlarge Image

Western Blot - Anti-Keratin 8 (phospho Ser432) Antibody (A94572)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30' using Anti-Keratin 8 (phospho Ser432) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Keratin 8 (phospho Ser432) Antibody (A94572)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-Keratin 8 (phospho Ser432) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Keratin 8 (phospho Ser432) Antibody (A94572)

Immunofluorescence analysis of HeLa cells using Anti-Keratin 8 (phospho Ser432) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-Keratin 8 (phospho Ser432) Antibody (A0883) - Antibodies.comEnlarge Image

Western Blot - Anti-Keratin 8 (phospho Ser432) Antibody (A94572)

Western blot analysis of 293 cells using Anti-Keratin 8 (phospho Ser432) Antibody.

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Proteins predicted to interact with Cytokeratin 8

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
86.9% Relevancy Score
84.5% Relevancy Score
82.6% Relevancy Score
79.8% Relevancy Score
79.6% Relevancy Score
79.1% Relevancy Score
78.3% Relevancy Score
76.3% Relevancy Score