Mouse monoclonal [KN-03] antibody to Kinesin Heavy Chain.
Specificity
The antibody recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody recognizes kinesin bound to taxol-stabilized microtubules.
Applications
ICC
Reactivity
Porcine, Rat, Mouse, Human
Immunogen
Enriched fraction of porcine brain kinesin.
Host
Mouse
Clonality
Monoclonal
Clone ID
KN-03
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Anti-Kinesin Heavy Chain Antibody [KN-03] (A85475)
Immunofluorescence staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using Anti-Kinesin Heavy Chain Antibody [KN-03] (A85475). Nuclei were stained with DAPI (blue).
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