This Lactate Assay Kit is a quantitative assay kit for measuring Lactate in animal and plant tissues, cells, plasma, serum, and other liquid samples.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Lactate is oxidized in a reaction coupled to WST-8 reduction, producing a colored formazan dye. The absorbance at 450 nm is proportional to lactate concentration.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, plasma, serum, and liquid samples
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Representative standard curve for Lactate Assay Kit (A319694). The y-axis is OD450 and the x-axis is lactate concentration (mM).
Customer Reviews (1)
Verified Customer
Antreas Ermogenous, Democritus University of Thrace
Verified Customer
Applications: Lactate Quantification in Cell Culture Media Using Colorimetric Assay
Species: Human
Sample: Human Colorectal Adenocarcinoma Cell Line HT29 and HT29 Cells Overexpressing ALDH1B1
Experimental Conditions: HT29 and HT29/ALDH1B1 seeded at a density of 1 × 105 cells per well in six-well plates (triplicates) and maintained at 37°C under 5% CO2. Culture media were collected every 24 hours for five consecutive days, centrifuged at 12,000 × g for 10 minutes at 4°C and stored at -80oC until analysis. Lactate levels were quantified according to the manufacturer's protocol.
Results Summary: Absorbance values increased progressively and reached saturation after day 3 in both cell lines
Comments: Very quick response to a small mistake in the volume of one reagent, and they quickly fixed the issue by sending the correct one for free.
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