LAG-3 expression in Human Lymph Node (A) and Tonsil (B) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed for 1 hour with Anti-LAG-3 Antibody (A82528) at 1µg/ml (A) or 0.5µg/ml (B) and detected by chemiluminescence.
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