Anti-LCK Antibody [LCK-01] (A86727)

Name: Anti-LCK Antibody [LCK-01]
Brand: Antibodies.com
SKU: A86727
Price: 125.0 GBP
Availability: InStock

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$210

Mouse monoclonal [LCK-01] antibody to LCK for Flow Cytometry (Intracellular), IP, WB and ICC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Plasma Membrane Marker

Specifications

Name

Anti-LCK Antibody [LCK-01]

Description

Mouse monoclonal [LCK-01] antibody to LCK.

Specificity

This antibody recognizes defined epitope (amino acids 22-36) of LCK, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen).

Applications

Flow Cytometry (Intracellular), IP, WB, ICC

Dilutions

Flow Cytometry: 1-5 µg/ml; Intracellular staining.

Reactivity

Human

Cross Reactivity

This antibody does not cross react with Mouse.

Immunogen

Peptide corresponding to amino acids 22-36 in the sequence of human Lck.

Host

Mouse

Clonality

Monoclonal

Clone ID

LCK-01

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Predicted MW

56 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, Tyrosine-protein kinase Lck

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Anti-LCK Antibody [LCK-01] (A86727)

Western blotting analysis (reducing conditions) of human Lck in whole cell lysate using Anti-LCK Antibody [LCK-01] (A86727). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with LckLane 2: HEK-293T cell line transfected with LckLane 3: HEK-293T cell line (non-transfected)

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Flow Cytometry - Anti-LCK Antibody [LCK-01] (A86727)

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using Anti-LCK Antibody [LCK-01] (A86727), (concentration in sample 9 µg/ml, GAM APC) and Anti-CD3 Antibody [UCHT1] (Pacific Blue), (20 µl reagent / 100 µl of peripheral whole blood).

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Flow Cytometry - Anti-LCK Antibody [LCK-01] (A86727)

Separation of human CD3 positive LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained with Anti-LCK Antibody [LCK-01] (A86727), (concentration in sample 9 µg/ml, GAM APC).

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Flow Cytometry - Anti-LCK Antibody [LCK-01] (A86727)

Flow cytometry intracellular staining of human peripheral whole blood using Anti-LCK Antibody [LCK-01] (A86727), (concentration in sample 9 µg/ml, GAM APC).

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