Rabbit polyclonal antibody to LIMK1 (Phospho-Thr508)
Specificity
The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from UV-treated C2C12, PMA-treated Hela and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508) Antibody #11126.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508) Antibody #11126 (left) or the same antibody preincubated with blocking peptide (right).
