This kit enables simultaneous detection of live and dead cells using fluorescent dyes, allowing rapid assessment of cell viability by fluorescence microscopy or flow cytometry.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit uses a membrane-permeable green fluorescent dye to label live cells and a membrane-impermeable red fluorescent dye to label dead cells. Simultaneous staining enables direct assessment of cell viability based on membrane integrity and intracellular esterase activity.
Platform
Flow Cytometer, Fluorescence Microscope
Sample Type
Cultured cells
Assay Time
15-30 minutes
Components
LiveDye, NucleiDye, Assay Buffer (10X)
Storage
Shipped at 4°C. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
The red dye is a potential mutagen. Take appropriate precautions when handling this reagent.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunocytochemistry - Live and Dead Cell Double Staining Kit (A319650)
Hela cells stained with Live and Dead Cell Double Staining Kit (A319650). A: Live cells stain green, B: Dead cells (30 minute incubation in 70% ethanol) stain red.
