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Anti-MCM7/PRL Antibody (A13300)

Western Blot - Anti-MCM7/PRL Antibody (A13300) - Antibodies.com Immunofluorescence - Anti-MCM7/PRL Antibody (A13300) - Antibodies.com Western Blot - Anti-MCM7/PRL Antibody (A13300) - Antibodies.com
$375
Product Datasheet

Rabbit polyclonal antibody to MCM7/PRL for WB, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-MCM7/PRL Antibody
Description
Rabbit polyclonal antibody to MCM7/PRL.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MCM7 (NP_005907.3).
Sequence
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
81 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-MCM7/PRL Antibody (A13300) - Antibodies.comEnlarge Image

Western Blot - Anti-MCM7/PRL Antibody (A13300)

Western blot analysis of extracts of various cell lines, using Anti-MCM7/PRL Antibody (A13300) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-MCM7/PRL Antibody (A13300) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-MCM7/PRL Antibody (A13300)

Immunofluorescence analysis of U-2 OS cells using Anti-MCM7/PRL Antibody (A13300) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-MCM7/PRL Antibody (A13300) - Antibodies.comEnlarge Image

Western Blot - Anti-MCM7/PRL Antibody (A13300)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-MCM7/PRL Antibody (A13300). This Western blot was performed on the immunoprecipitate using Anti-MCM7/PRL Antibody (A13300) at a dilution of 1:500.
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