Rabbit polyclonal antibody to MNK1 (Phospho-Thr255)
Specificity
The antibody detects endogenous levels of MNK1 only when phosphorylated at threonine 255.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 255 (L-T-T(p)-P-C) derived from Human MNK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody #12142. The lane on the right is treated with the synthesized peptide.
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