Principle of Assay
Stable chromogen/substrate solution to be used in conjunction with peroxidase-based immunostaining systems. Mono AEC/Plus is a single stable solution, therefore the solution is immediately ready for use. Thus, time is saved from preparing a working solution. As compared to typical DAB Substrate/Chromogen solutions, this single chromogen/substrate solution can be used up until the expiration date. Unused solution does not need to be immediately discarded, reducing waste. Peroxidase reacts with hydrogen peroxide substrate to degrade it, which reacts with AEC, precipitating it at positive sites yielding a reddish brown colored product.
