Mouse GRP78 BiP ELISA Kit (A310106)

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This product is discontinued

Mouse GRP78 BiP ELISA Kit (A310106) has been discontinued and is no longer available.

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Specifications
Components

Name

Mouse GRP78 BiP ELISA Kit

Description

Mouse GRP78 BiP ELISA Kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse GRP78 BiP in serum, plasma, and other biological fluids.

Assay Type

Sandwich (quantitative)

Principle of Assay

Mouse GRP78 BiP ELISA Kit (A310106) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse GRP78 BiP in serum, plasma or other biological fluids. An antibody specific for GRP78 BiP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GRP78 BiP present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-GRP78 BiP Antibody, which also binds the GRP78 BiP present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-GRP78 BiP Antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-GRP78 BiP Antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of GRP78 BiP captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of GRP78 BiP in the samples can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Platform

Pre-coated Microplate (12 x 8 well strips)

Detection Type

Colorimetric

Instrument

Colorimetric Microplate Reader

Sample Type

Serum, plasma or other biological fluids.

Sensitivity

0.121 ng/ml

Product Range

2-32 ng/mL

Assay Time

1h 30m

Reactivity

Mouse

Storage

Store at +4°C. Do not use past expiration date!

Synonyms

78 kDa glucose regulated protein, 78 kDa glucose-regulated protein, AL022860, AU019543, BIP, D2Wsu141e, D2Wsu17e, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, Epididymis secretory sperm binding protein Li 89n, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, GRP-78, GRP78, GRP78_HUMAN, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, Heat shock protein family A (Hsp70) member 5, HEL S 89n, Hsce70, HSPA 5, HSPA5, Immunoglobulin Heavy Chain Binding Protein, Immunoglobulin heavy chain-binding protein, mBiP, MIF2, Sez7

Product Note

Information online is representative. Always refer to the Product Manual inside the kit.

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Kit Components

Item	Quantity	Storage
Pre-Coated 96 Well Microplate	12 x 8 Well Strips	+4°C
Standard Solution	500µl	+4°C
Standard Diluent	3ml	+4°C
Biotinylated Detection Antibody	1ml	+4°C
Streptavidin-HRP	6ml	+4°C
Wash Buffer (25X)	20ml	+4°C
Substrate Solution A	6ml	+4°C
Substrate Solution B	6ml	+4°C
Stop Solution	6ml	+4°C
Plate Sealers	5 Adhesive Strips	-
Foil Pouch	1 Zip-Sealed Pouch	-

Required Materials

The following materials are not included in the kit, but are required to run this assay:

Microplate reader capable of measuring absorbance at 450nm ± 10nm.
Squirt bottle, multichannel pipette reservoir, or automated microplate washer.
Graph paper or computer software capable of generating logarithmic functions.
Test tubes and Eppendorf tubes to prepare standards and samples.
Multi- and single-channel pipettes and sterile pipette tips.
Deionized or distilled water.
Absorbent paper towels.
37°C incubator.

Important Notes

Samples cannot be diluted for use with this kit. Owing to the material used to prepare the kit, sample matrix interference may falsely depress the specificity and accuracy of the assay.
Sample concentrations should be estimated before being used in the assay. If the sample concentration is not within the range of the standard curve, users must contact us to determine the optimal sample for their experiments.
Samples containing Sodium Azide (NaN₃) cannot be used in this assay. NaN₃ inhibits the activity of Horseradish Peroxidase (HRP) which is used in the detection step.
This ELISA kit is intended for the analysis of biological samples and has not been validated for purified/recombinant proteins, industrial, or commercial materials. Performance with such samples cannot be guaranteed, as differences in expression, sequence, tags, folding, post-translational modifications, purity, activity, or buffer composition may affect assay results. For further guidance, please contact our technical support team.