Mouse Monocyte Chemoattractant Protein 1 ELISA Kit (A33049)

Mouse Monocyte Chemoattractant Protein 1 ELISA Kit

Mouse Monocyte Chemoattractant Protein 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Monocyte Chemoattractant Protein-1 in serum, cell culture supernatant and other biological fluids.

Sandwich (quantitative)

This mouse MCP-1 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse MCP-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse MCP-1, if present, will bind and become immobilized by the antibody pre-coated on the wells. A biotin conjugated antibody specific for mouse MCP-1 is added to each well. The biotin conjugated antibody will bind to the mouse MCP-1 on the plate. The microtiter plate wells are thoroughly washed to remove unbound biotin conjugate and other components of the sample. Avidin has a very high affinity to biotin. In order to quantify the amount of mouse MCP-1 present in the sample, a standardized preparation of avidin conjugated horseradish peroxidase (HRP) is added to each well. Avidin-HRP will bind to the biotin on plate during incubation. The wells are thoroughly washed to remove all unbound Avidin-HRP conjugate and a TMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells to which mouse MCP-1, Biotin conjugate and Avidin-HRP are attached will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

In order to measure the concentration of mouse MCP-1 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus MCP-1 concentration (pg/mL). The concentration of MCP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Colorimetric

Colorimetric Microplate Reader

Serum, cell culture supernatant and other biological fluids.

< 1.7 pg/ml

7.8-500 pg/ml

3h 30m

Mouse

Shipped and stored at 4°C.

C-C motif chemokine 2, C-C motif chemokine ligand 2, Ccl2, chemokine (C-C motif) ligand 2, Chemokine CC-2, GDCF-2, HC11, HSMCR30, Immediate-early serum-responsive protein JE, Je, MCAF, MCP-1, Mcp1, Monocyte chemoattractant protein 1, monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, monocyte secretory protein JE, Scya2, Sigje, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, Small-inducible cytokine A2, SMC-CF

Information online is representative. Always refer to the Product Manual inside the kit.