Principle of Assay
The Mouse on Mouse HRP/DAB Detection Kit is a non-biotin, one-step detection system suitable for detecting mouse antigens on mouse tissue sections. The Mouse on Mouse HRP/DAB Detection Kit may be used with formalin-fixed paraffin embedded and cryostat sections, as well as blood smears, cytosmears, and cell preparations. The Mouse on Mouse HRP/DAB Detection Kit has been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step Mouse on Mouse HRP/DAB Detection Kit enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. The Mouse on Mouse HRP/DAB Detection Kit is suitable for use with mouse IgG and IgM antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining instruments and is well suited for multiplex immunohistochemical staining assays.
