Principle of Assay
The Mouse/Rabbit HRP Kit is suitable for use with mouse and rabbit IgG and IgM antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining platforms. The Mouse/Rabbit HRP Kit is a non-biotin, one-step detection system suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The Mouse/Rabbit HRP Kit may also be used with blood smears, cytosmears, and cell preparations. The Mouse/Rabbit HRP Kit has been developed by directly labeling anti-mouse and anti-rabbit immunoglobulins with enzymes using a proprietary tandem hyper-labelling technology. This ensures consistent and reproducible immunodetection of mouse and rabbit antibodies with a single reagent. Nuclear, cytoplasmic and membrane antigens in different types of tissues can be detected readily. The single step Mouse/Rabbit HRP Kit enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, avidin/streptavidin conjugates, with significantly lower background.
