Rabbit polyclonal antibody to n-NOS (Phospho-Ser852)
Specificity
The antibody detects endogenous level of n-NOS only when phosphorylated at serine 852.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 852(F-N-S(p)-V-S) derived from Human n-NOS .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
bNOS, Constitutive NOS, NC-NOS, Neuronal NOS, Nitric oxide synthase 1, Nitric oxide synthase, brain, NOS type I, NOS1, Peptidyl-cysteine S-nitrosylase NOS1
Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) antibody #11975.The lane on the right is treated with the antigen-specific peptide.
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