This NAD(H) Assay Kit is a quantitative assay kit for measuring NAD/NADH in animal tissues, plant tissues, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This assay uses an enzyme cycling reaction in which NADH reduces WST-8 to a colored formazan product. Absorbance at 450 nm is proportional to the total amount of NAD+ and NADH in the sample.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, bacteria, serum, and plasma
Assay Time
40-80 minutes
Components
Assay Buffer, EtOH Solution, WST-8 Solution, Enhancer, NAD Cycling Enzyme Mix, NAD Standard (10 mM), NAD Extraction Buffer, NADH Extraction Buffer
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
