This NAD+ kinase Activity Assay Kit is a quantitative assay kit for measuring NADK activity in serum, plasma, animal and plant tissues, cells, and bacteria.
NAD+ kinase phosphorylates NAD+ to generate NADP+, which is subsequently reduced to NADPH in a coupled reaction. Activity is determined from NADPH formation measured at 340 nm.
Platform
96-Well Microplate, Microcuvette
Sample Type
Serum, plasma, animal tissues, plant tissues, cells, and bacteria
Assay Time
30-60 minutes
Components
Extraction Buffer, Assay Buffer I, Assay Buffer II, Substrate Mix, Enzyme Mix
Storage
Shipped at 4°C. Store at -20°C in the dark. Do not use past expiration date!
General Notes
A 96-well clear flat bottom UV-transparent microplate or microquartz cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using NAD+ kinase Activity Assay Kit (A319708)? Please let us know so that we can list the citation on this page.
