Mouse monoclonal [NF-01] antibody to Neurofilament H.
Specificity
The antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
Applications
IHC-P, WB, ICC
Dilutions
IHC-P: 5-10 µg/ml, WB: 1-2 µg/ml.
Reactivity
Mammalian
Immunogen
Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Host
Mouse
Clonality
Monoclonal
Clone ID
NF-01
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
210 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blotting analysis of human neurofilament H protein using Anti-Neurofilament H Antibody [NF-01] (A85597) on lysates of HEK-293 cell line, human brain lysate, and MCF-7 cell line (neurofilament non-expressing cell line; negative control) under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-neurofilament H monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Neurofilament H was detected around 180 kDa.
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