Mouse monoclonal [NF-05] antibody to Neurofilament H.
Specificity
The antibody recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.
Applications
WB, IHC-Fr, ELISA
Dilutions
ELISA: This antibody has been tested as the capture antibody, WB: 1-2 µg/ml.
Reactivity
Human, Rat, Porcine
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules.
Host
Mouse
Clonality
Monoclonal
Clone ID
NF-05
Isotype
IgG1
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
210 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western Blot - Anti-Neurofilament H Antibody [NF-05] (A85669)
Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by Anti-Neurofilament Heavy Protein Antibody (A85669).
Publishing research using Anti-Neurofilament H Antibody [NF-05] (A85669)? Please let us know so that we can list the citation on this page.
![Western Blot - Anti-Neurofilament H Antibody [NF-05] (A85668) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85669_145.jpg?profile=product_top)
![Western Blot - Anti-Neurofilament H Antibody [NF-05] (A85668) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85669_145.jpg?profile=product_top_thumb)
![Western Blot - Anti-Neurofilament H Antibody [NF-05] (A85668) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85669_145.jpg?profile=product_image)