Western Blot - Anti-NFkB p100 / NFKB2 Antibody (A93099)
Western blot analysis of extracts from normal (control) and NF-κB2 knockout (KO) HeLa cells, using Anti-NFkB p100 / NFKB2 Antibody (A93099) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.