Rabbit polyclonal antibody to NFkB-p65 (Phospho-Ser529)
Specificity
The antibody detects endogenous level of NFkB-p65 only when phosphorylated at serine 529.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 529 (L-L-S(p)-G-D) derived from Human NFkB-p65.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
65kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65 (Phospho-Ser529) Antibody #11217 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody #11217.
