Rabbit polyclonal antibody to NFkB-p65 (Phospho-Thr254)
Specificity
The antibody detects endogenous level of NF-kB p65 only when phosphorylated at threonine 254.
Applications
ELISA, WB, ICC/IF, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFkB-p65.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p65 (Phospho-Thr254) Antibody #11010 (left) or the same antibody preincubated with blocking peptide (right).
