This Nitric Oxide Assay Kit is a quantitative assay kit for measuring Nitric Oxide activity in animal and plant tissues, cells, plasma, serum, urine, and other biological fluids.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
Nitric oxide is quantified indirectly after reduction of nitrate to nitrite using an improved Griess reaction. Nitrite forms an azo dye that is measured colorimetrically at 540 nm.
NaNO2 Standard (1M), Griess Reagent I, Griess Reagent II, VCl3 Reagent, ZnSO4
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
A 96-well microplate or microglass cuvette is required for this assay.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.