OneMark 100 with Novel Green is designed to show virtually uniform spacing over a wide fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking dyes. Optimized for direct loading onto unstained agarose gels. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes, Ethidium Bromide or SYBR® Green I. A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes. Phenol-extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Covers the size range 100-3,000 base pairs. Number of bands = 12. The 500 and 1,500 base pair bands have increased intensity to act as reference points. Contains Bromophenol Blue and Xylene Cyanol FF as the tracking dyes.
Applications
Agarose gel electrophoresis
Recommended Dilutions
Load 6 µl/well
Concentration
90 µg/ml
Product Form
Liquid
Formulation
10 mM Tris-HCl pH 8.0, 1 mM EDTA.
Storage
Shipped at 4°C. Store at room temperature or 4°C for up to 6 months and -20°C for up to 12 months.
General Notes
This product is light-sensitive and should be stored protected from light.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
The DNA ladder contains fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to act as reference points. The approximate mass of each band is provided (0.54 µg load) to aid quantitation of sample bands of similar size and intensity. Visualized using a blue-light transilluminator.
Publishing research using OneMARK 100 (Ready-to-Use) (A318975)? Please let us know so that we can list the citation on this page.
