Mouse monoclonal [C-11] antibody to pan Cytokeratin.
Specificity
This antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, and 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using Anti-pan Cytokeratin Antibody [C-11] (A86447).
Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained with Anti-pan Cytokeratin Antibody [C-11] (A86447), (concentration in sample 3 µg/ml, GAM APC).
Flow cytometry intracellular staining of human peripheral whole blood spiked with MCF-7 cells staining pan Cytokeratin with Anti-pan Cytokeratin Antibody [C-11] (A86447), (concentration in sample 3 µg/ml, GAM APC).
Western blotting analysis of human cytokeratins using Anti-pan Cytokeratin Antibody [C-11] (A86447) on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.
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