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Phosphoenolpyruvate Carboxykinase / PEPCK Activity Assay Kit (A319715)

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$280
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Phosphoenolpyruvate Carboxykinase / PEPCK Activity Assay Kit
Description
This Phosphoenolpyruvate Carboxykinase / PEPCK Activity Assay Kit is a quantitative assay kit for measuring Phosphoenolpyruvate Carboxykinase / PEPCK activity in animal tissue, plant tissue, cells, bacteria, serum, and plasma.
Applications
Functional Studies
Assay Type
Quantitative
Principle of Assay
This kit provides a simple, convenient and rapid method for phosphoenolpyruvate carboxykinase (PEPCK) activity detection, which is suitable for testing animal and plant tissues, cells, bacteria, serum (plasma), and other samples. The principle is that PEPCK catalyzes the oxidation of oxaloacetate to produce phosphoenolpyruvate and carbon dioxide. Pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to produce NAD+. The rate of NADH decline is measured at 340 nm, and reflects PEPCK activity.
Platform
96-Well Microplate, Microcuvette
Sample Type
Animal tissues, plant tissues, cells, bacteria, serum, and plasma.
Components
Extraction Buffer, Reagent I, Reagent II, Reagent III, Reagent IV, Reagent V
Storage
Shipped at 4°C. Store at -20°C in the dark. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!
General Notes
96-well clear flat bottom UV-transparent microplate / microquartz cuvette is required.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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