Anti-Phosphotyrosine Antibody [P-Tyr-01] (A86791) Specificity Verification by Flow Cytometry. Anti-Phosphotyrosine purified antibody (concentration in sample 2 µg/ml, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 kappa Isotype Control [MOPC-21] (A86202) under same conditions (concentration in sample 2 µg/ml, GAM FITC, black-dashed histogram).
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