The antibody detects endogenous level of total PIAS1 antibody.
Applications
WB, ICC/IF
Dilutions
Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Reactivity
Human, Mouse
Immunogen
Recombinant protein of human PIAS1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were purified by affinity purification using immunogen.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, E3 SUMO-protein transferase PIAS1, GBP, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using PIAS1 antibody. Green£ºGFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
