Rabbit polyclonal antibody to PKA a / ß CAT (Phospho-Thr197)
Specificity
The antibody detects endogenous levels of PKA α / β CAT only when phosphorylated at threonine 197.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA a / ß CAT.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Anti-PKA a / ß CAT (Phospho-Thr197) Antibody (A50140)
Western blot analysis of extracts from mouse brain cells using PKA α/β CAT (Phospho-Thr197) Antibody #11667.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemistry - Anti-PKA a / ß CAT (Phospho-Thr197) Antibody (A50140)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody #11667 (left)or the same antibody preincubated with blocking peptide (right).
