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Anti-PRAMEF20 Antibody (A90492)

Western Blot - Anti-PRAMEF20 Antibody (A90492) - Antibodies.com Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492) - Antibodies.com Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492) - Antibodies.com Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492) - Antibodies.com
This product is discontinued

Anti-PRAMEF20 Antibody (A90492) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-PRAMEF20 Antibody
Description
Rabbit polyclonal antibody to PRAMEF20.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1).
Sequence
MSSKPLLTLQELAAQNILRNEALAISALQYLPELFLPQLLKQAYDGNHLKVLRAIVSSWPFPRLPLGALKKRTPYLETQLQVVLEEVDKLLIQEVHPREYKLEVLDLRSVGQNYLNVWPGAMDDWLPKTTQTDPCCSKTVMTQPLKVLVDLQLNNRGQSRLFSYLVQWSDRRKGLLQLYCNKLQIWSPSQQNYRKLSQKVNLDYVETLGLHSSCSPSFLLNFAPYLRQMRNLRKLALSNIREEHIISPEKRRCIITRFTLQILKLESLQKLHLDAVCFLEGHLDQLLGSVKTSLDELAVTHCNLSVSEWSHFSEFPCVSQLQQLNLENVRLTGLSPEPLQVLLVKAGPTLVALDLEDCHLEDGQLYAILPALSKCFKLTKFSFYGNQISMLAMKDLLHHTASLIHLRLELYPVPQDSYDYHGTPLMQRMQQHCDELMNLLKTFRNPGRVFFGTDRCDQCCNRYIYNKTIMCNCRRSY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-PRAMEF20 Antibody (A90492) - Antibodies.comEnlarge Image

Western Blot - Anti-PRAMEF20 Antibody (A90492)

Western blot analysis of extracts of various cell lines, using Anti-PRAMEF20 Antibody (A90492) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PRAMEF20 Antibody (A90492) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-PRAMEF20 Antibody (A90492) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PRAMEF20 Antibody (A90492)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PRAMEF20 Antibody (A90492) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.