Rabbit polyclonal antibody to Proteasome α3 (Phospho-Ser250)
Specificity
The antibody detects endogenous levels of Proteasome α3 only when phosphorylated at serine 250.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 250 (D-E-S(p)-D-D) derived from Human Proteasome α3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 30mins), using Proteasome α3 (Phospho-Ser250) antibody #12113. The lane on the right is treated with the synthesized peptide.
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