Principle of Assay
This kit was specially developed for the detection of pyruvic acid in a variety of biological samples such as plasma, serum, animal and plant tissues, cells, and bacteria. The operation is simple and convenient, and the detection is sensitive and accurate. Pyruvic acid reacts with 2,4-dinitrophenylhydrazine to produce a red compound with a maximum absorption peak at 520 nm. Within a certain concentration range, the pyruvic acid content has a linear relationship with the absorbance at 520 nm. The pyruvic acid content of the sample is calculated from a standard curve..
